Type II Collagen Research Summary

The study aimed to assess the safety and efficacy of different dosages of orally administered type II collagen (CII) in patients with rheumatoid arthritis (RA). A total of 274 patients with active RA were recruited and randomized to receive a placebo or one of four dosages (20, 100, 500, or 2,500 micrograms/day) of oral CII for 24 weeks. The results showed that the group receiving the lowest dosage (20 micrograms/day) had a significant improvement according to the Paulus criteria compared to the placebo group. Patients with serum antibodies to CII at the start of the study were more likely to respond to the treatment, and no adverse events related to the treatment were detected.

The study concludes that the lowest dosage of CII had positive effects on RA patients, and the presence of serum antibodies to CII could predict a better response to the therapy. Further studies are needed to fully evaluate the effectiveness of this therapeutic approach.

Another research study investigated the impact of prolyl-hydroxyproline (Pro-Hyp), a collagen-derived peptide found in human blood after consuming collagen, on the migration and proliferation of mouse skin fibroblasts. The findings imply that Pro-Hyp may activate fibroblast growth in the skin, leading to an enhanced migration of these cells from the skin tissue.

The research examines the effects of a water-soluble undenatured type II collagen (NEXT-II) on collagen-induced arthritis (CIA) in mice. The study found that oral administration of NEXT-II significantly reduced arthritis severity and serum IL-6 levels while increasing IL-2 levels and the proportion of regulatory T cells (Tregs) in the mice. These results suggest that NEXT-II effectively suppresses CIA by inducing regulatory T cells when administered orally.

References:

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2. Identification of food-derived collagen peptides in human blood after oral ingestion of gelatin hydrolysates
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4. Increase in bone mineral density through oral administration of shark gelatin to ovariectomized rats
5. Chemical, biochemical, functional, and nutritional characteristics of collagen in food systems
6. c-Jun-dependent inhibition of cutaneous procollagen transcription following ultraviolet irradiation is reversed by all-trans retinoic acid
7. Disease-dependent local IL-10 production ameliorates collagen induced arthritis in mice
8. IL-10-producing regulatory B10 cells ameliorate collagen-induced arthritis via suppressing Th17 cell generation
9. Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia
10. Treatment of rheumatoid arthritis with oral type II collagen. Results of a multicenter, double-blind, placebo-controlled trial
11. Blood and synovial fluid cytokine signatures in patients with juvenile idiopathic arthritis: a cross-sectional study
12. Biosynthesis of collagen and other matrix proteins by articular cartilage in experimental osteoarthrosis
13. Effects of orally administered undenatured type II collagen against arthritic inflammatory diseases: a mechanistic exploration
14. CD4+ CD25+ T cells with the phenotypic and functional characteristics of regulatory T cells are enriched in the synovial fluid of patients with rheumatoid arthritis
15. Effects of Pro-Hyp, a collagen hydrolysate-derived peptide, on hyaluronic acid synthesis using in vitro cultured synovium cells and oral ingestion of collagen hydrolysates in a guinea pig model of osteoarthritis
16. Effect of Prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin
17. Water-soluble undenatured type II collagen ameliorates collagen-induced arthritis in mice
18. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients
19. Production of interleukin-7 and interleukin-15 by fibroblast-like synoviocytes from patients with rheumatoid arthritis
20. Central penetration and stability of N-terminal tripeptide of insulin-like growth factor-I, glycine-proline-glutamate in adult rat
21. Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling
22. Urinary collagen type II C-telopeptide fragments are sensitive markers of matrix metalloproteinase-dependent cartilage degradation in rat adjuvant-induced arthritis
23. Treating arthritis by immunomodulation: is there a role for regulatory T cells?
24. TGF beta regulation of cell proliferation
25. Suppression of collagen-induced arthritis by oral administration of transgenic rice seeds expressing altered peptide ligands of type II collagen
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27. Recent progress toward biomarker identification in osteoarthritis
28. Undenatured type II collagen (UC-II®) for joint support: a randomized, double-blind, placebo-controlled study in healthy volunteers
29. Modulation of tryptophan catabolism by regulatory T cells
30. The amino acid composition of gelatins, collagens and elastins from different sources
31. Type II collagen serology: a guide to clinical responsiveness to oral tolerance?
32. Differentiation and cell surface expression of transforming growth factor-beta receptors are regulated by interaction with matrix collagen in murine osteoblastic cells
33. Goblet cells deliver luminal antigen to CD103+ dendritic cells in the small intestine
34. Assessment of effectiveness of oral administration of collagen peptide on bone metabolism in growing and mature rats
35. Denaturation of collagen via heating: an irreversible rate process
36. Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis
37. Effects of oral administration of type II collagen on rheumatoid arthritis
38. How tolerogenic dendritic cells induce regulatory T cells
39. Suppression of immune induction of collagen-induced arthritis in IL-17-deficient mice
40. Modulation by cytokines of induction of oral tolerance to type II collagen
41. Safety and toxicological evaluation of a novel, water-soluble undenatured type II collagen
42. Safety and efficacy of undenatured type II collagen in the treatment of osteoarthritis of the knee: a clinical trial
43. Collagen peptides enhance hippocampal neurogenesis and reduce anxiety related behavior in mice
44. Low dose native type II collagen prevents pain in a rat osteoarthritis model
45. Oral tolerance and OVA-induced tolerogenic dendritic cells reduce the severity of collagen/ovalbumin-induced arthritis in mice
46. Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer's patches in an orally tolerized, collagen-induced arthritis mouse model
47. Role of collagen hydrolysate in bone and joint disease
48. Collagen of articular cartilage
49. Molecular basis of the alteration in skin collagen metabolism in response to in vivo dexamethasone treatment: effects on the synthesis of collagen type I and III, collagenase, and tissue inhibitors of metalloproteinases
50. Oral administration of type-II collagen peptide 250-270 suppresses specific cellular and humoral immune response in collagen-induced arthritis
51. Chicken type II collagen induced immune balance of main subtype of helper T cells in mesenteric lymph node lymphocytes in rats with collagen-induced arthritis
52. Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen
53. Differentiation and transforming growth factor-beta receptor down-regulation by collagen-alpha2beta1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells
54. Low molecular weight bioactive peptides derived from the enzymatic hydrolysis of collagen after isoelectric solubilization/precipitation process of turkey by-products
55. Molecular basis of sun-induced premature skin ageing and retinoid antagonism
56. Collagen-derived dipeptide, proline-hydroxyproline, stimulates cell proliferation and hyaluronic acid synthesis in cultured human dermal fibroblasts
57. The role of dendritic cell subsets in selection between tolerance and immunity
58. Ingested hyaluronan moisturizes dry skin
59. Ingestion of BioCell Collagen(®), a novel hydrolyzed chicken sternal cartilage extract; enhanced blood microcirculation and reduced facial aging signs
60. Safety and toxicological evaluation of undenatured type II collagen
61. Peptide mimetics of neurotrophins and their receptors
62. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis